How Do You Spell BAMHI DEOXYRIBONUCLEASE?

Pronunciation: [bˈam ˌe͡ɪt͡ʃˈa͡ɪ diːˈɒksɪɹˌɪbənjˌuːklɪˌe͡ɪs] (IPA)

The spelling of the word "BamHI Deoxyribonuclease" can be tricky for those unfamiliar with the IPA phonetic transcription used to represent the pronunciation of words. The first part of the word, "BamHI," is pronounced /ˌbæmˌeɪtʃˈaɪ/. The second part, "Deoxyribonuclease," is pronounced /diˌɑksiˌraɪboʊˈnuːkleɪz/. The use of scientific jargon and complex pronunciation can create a barrier for those not used to the terminology. Nevertheless, it is essential in discussing scientific research on DNA and molecular biology.

BAMHI DEOXYRIBONUCLEASE Meaning and Definition

1. BamHI Deoxyribonuclease is a type II restriction enzyme that is commonly used in molecular biology for manipulating and studying DNA. It is derived from the bacterium Bacillus amyloliquefaciens H and is typically obtained through recombinant DNA technology.

   This enzyme belongs to the endonuclease class, which means it cleaves DNA at specific recognition sequences. BamHI recognizes and cuts DNA at the palindromic sequence 5'-GGATCC-3', generating a double-stranded DNA fragment with cohesive ends. This characteristic makes BamHI especially useful in DNA cloning, as it can be used to generate compatible ends for the insertion of foreign DNA fragments into plasmids or other vectors.

   BamHI Deoxyribonuclease has high specificity, meaning it only cuts at its target recognition sequence. This specificity is essential for experimental accuracy, as it allows for precise manipulation of DNA fragments. Additionally, BamHI does not require ATP for its activity and functions optimally in a buffer solution at pH 7.4 and a temperature range of 37-45 degrees Celsius.

   The application of BamHI Deoxyribonuclease is vast and diverse. It is commonly used in DNA modification experiments, such as restriction mapping, gene cloning, gene expression studies, and recombinant DNA technology. Its use is critical in various disciplines, including molecular biology, genetics, biotechnology, and genetic engineering.

Common Misspellings for BAMHI DEOXYRIBONUCLEASE

• vamhi deoxyribonuclease
• namhi deoxyribonuclease
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• bamui deoxyribonuclease
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• bamhu deoxyribonuclease
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• bamhk deoxyribonuclease

Etymology of BAMHI DEOXYRIBONUCLEASE

The term "BamHI Deoxyribonuclease" is derived from the name of the restriction enzyme "BamHI" and the term "deoxyribonuclease".

The name "BamHI" refers to the location where the enzyme was first isolated, which is the bacterium Bacillus amyloliquefaciens strain H. The letters "Bam" represent the first three letters of the bacterium's name, and "H" refers to the restriction enzyme isolated from that strain.

"Deoxyribonuclease" is a compound word that describes an enzyme that breaks down or cleaves the DNA (deoxyribonucleic acid) molecule. The term "deoxy-" refers to the absence of an oxygen atom in the sugar backbone of DNA, and "ribonuclease" indicates the enzyme's ability to degrade ribonucleic acids.