ENDODEOXYRIBONUCLEASE HSAI Meaning and
Definition
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Endodeoxyribonuclease HsaI is a specific type II restriction enzyme. It is commonly used in molecular biology and genetic engineering research, particularly in DNA sequencing, cloning, and manipulation techniques. This enzyme is derived from the bacterium Haemophilus sputorum.
HsaI is an endodeoxyribonuclease, meaning it is an enzyme that cleaves DNA from within the molecule instead of at the ends. It recognizes specific DNA sequences and cuts the phosphodiester bonds within these sequences. HsaI specifically recognizes and cleaves the DNA sequence 5'-GRCGYC-3', where R represents either A or G, and Y represents either T or C. It generates blunt ends after the cleavage, resulting in DNA fragments devoid of overhanging single-stranded tails.
HsaI is an extensively studied restriction enzyme due to its high specificity and the presence of its recognition site in various DNA sequences found in different organisms. Researchers have used HsaI for DNA fingerprinting, gene mapping, and analyzing DNA-protein interactions. Additionally, HsaI is often used in combination with other restriction enzymes to generate unique DNA fragments for DNA sequencing or cloning purposes.
The study of HsaI and other restriction enzymes has significantly advanced our understanding of DNA structure, genetics, and the function of specific genes. These enzymes are fundamental tools in molecular biology laboratories, playing a crucial role in genetic analysis and manipulation techniques.
Common Misspellings for ENDODEOXYRIBONUCLEASE HSAI
- wndodeoxyribonuclease hsai
- sndodeoxyribonuclease hsai
- dndodeoxyribonuclease hsai
- rndodeoxyribonuclease hsai
- 4ndodeoxyribonuclease hsai
- 3ndodeoxyribonuclease hsai
- ebdodeoxyribonuclease hsai
- emdodeoxyribonuclease hsai
- ejdodeoxyribonuclease hsai
- ehdodeoxyribonuclease hsai
- ensodeoxyribonuclease hsai
- enxodeoxyribonuclease hsai
- encodeoxyribonuclease hsai
- enfodeoxyribonuclease hsai
- enrodeoxyribonuclease hsai
- eneodeoxyribonuclease hsai
- endideoxyribonuclease hsai
- endkdeoxyribonuclease hsai
- endldeoxyribonuclease hsai
- endpdeoxyribonuclease hsai
Etymology of ENDODEOXYRIBONUCLEASE HSAI
The word "Endodeoxyribonuclease HsaI" can be broken down into its components for a clearer understanding of its etymology:
1. Endodeoxyribonuclease: "Endo-" is a prefix meaning "within" or "inside", derived from the Greek word "endos". "Deoxyribonuclease" is a compound word involving "deoxyribose", a type of sugar, and "nuclease", an enzyme that breaks down nucleic acids (like DNA). Therefore, "Endodeoxyribonuclease" refers to an enzyme that acts on DNA from within or inside.
2. HsaI: This component refers to a specific restriction enzyme HsaI, which comes from the bacterium Haemophilus sanguineus. Restriction enzymes are proteins that recognize specific DNA sequences and cut the DNA at those sites.
