How Do You Spell SITE SPECIFIC MUTAGENESES?

Pronunciation: [sˈa͡ɪt spəsˈɪfɪk mjˈuːte͡ɪd͡ʒnˌɛsiːz] (IPA)

The term "site-specific mutagenesis" refers to a genetic engineering technique commonly used in scientific research. It involves changing the genetic code of a specific site, or region, within a DNA sequence. The word "mutagenesis" describes the process of creating mutations, or changes, in DNA. The spelling of the word is pronounced /saɪt spəˈsɪfɪk mjuːtədʒəˈniːsɪs/ using the International Phonetic Alphabet. The IPA shows the pronunciation of the word's consonants, vowels, stress, and intonation, providing a standardized way of representing spoken language for global comprehension.

SITE SPECIFIC MUTAGENESES Meaning and Definition

  1. Site-specific mutagenesis is a genetic engineering technique that involves the deliberate alteration or modification of a specific site or region within a DNA molecule, usually a gene or a DNA sequence of interest. This technique allows researchers to introduce specific changes or mutations into the DNA sequence in order to study the functional consequences of these alterations.

    Site-specific mutagenesis is typically carried out in vitro using a variety of methods, such as oligonucleotide-directed mutagenesis, polymerase chain reaction (PCR), or other nucleic acid manipulation techniques. These methods involve designing short synthetic DNA fragments, called primers or oligonucleotides, which contain the desired mutation(s) and are complementary to the target DNA sequence. The primers are then used in combination with DNA polymerases and other enzymes to selectively amplify and modify the target DNA region, incorporating the desired mutations.

    This technique has numerous applications in molecular biology and genetics, including the study of gene function, protein structure-function relationships, protein engineering, and the development of recombinant DNA-based therapies or biotechnological products. Site-specific mutagenesis can be used to create point mutations, insertions, deletions, or other alterations in the DNA sequence, allowing researchers to investigate the effects of specific changes on gene expression, protein folding or activity, interaction with other molecules, or cellular phenotype.

    Overall, site-specific mutagenesis is a powerful tool that enables researchers to explore the role of specific genetic alterations in biological systems and contributes to our understanding of gene function and the development of novel therapeutic strategies.

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Similar spelling word for SITE SPECIFIC MUTAGENESES

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